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以冬小麦强抗寒品种东农冬麦1号为试验材料,从其根中克隆膨胀素基因TaEXPB8、TaEXPB10,并分析其在低温条件下的表达情况,为研究膨胀素基因与植物抗寒性之间的关系奠定基础。结果表明,在冬小麦强抗寒品种东农冬麦1号根中克隆得到膨胀素基因TaEXPB8、TaEXPB10,二者分别编码273、271个氨基酸,二者序列与Gen Bank上公布的序列同源性分别达到99.2%、99.6%。4℃、-10℃和-20℃的低温胁迫处理均可诱导TaEXPB8、TaEXPB10基因在冬小麦强抗寒品种东农冬麦1号根中表达,尤其是TaEXPB10基因,其受诱导程度高于TaEXPB8;两基因在低抗寒品种济麦22号中总体上不受诱导,甚至受到抑制。综合比较发现,4℃、-10℃和-20℃低温胁迫下,TaEXPB8、TaEXPB10基因在强抗寒品种东农冬麦1号中的表达量均明显高于低抗寒品种济麦22号。据此推测膨胀素基因TaEXPB8、TaEXPB10与冬小麦根系的低温胁迫耐受能力具有重要的相关性,有助于进一步深入了解冬小麦根系的抗寒机制。
In order to study the expression of swollenin genes TaEXPB8 and TaEXPB10 in their roots under the condition of low temperature, Dongnong Dongmai 1, a winter wheat cultivar with strong resistance to cold, The relationship between the foundation. The results showed that the swollenin genes TaEXPB8 and TaEXPB10 were cloned from the winter wheat cultivar Dongnong Dongmai No.1, respectively, and they encoded 273,271 amino acids respectively. The two sequences were homologous to those published on Gen Bank Reached 99.2%, 99.6%. TaEXPB8 and TaEXPB10 genes were induced at 4 ℃, -10 ℃ and -20 ℃ under low temperature stress. TaEXPB10 gene was expressed more strongly in winter wheat than in TaEXPB8, especially TaEXPB10. The two genes were not induced or even inhibited in low temperature resistant variety Jimai22. The results showed that the expression of TaEXPB8 and TaEXPB10 genes in Dongnong Dongmai 1, a strong winter wheat variety, was significantly higher than that in low-temperature-resistant variety Jimai 22 at low temperature of 4 ℃, -10 ℃ and -20 ℃. Therefore, it is speculated that the expansin genes TaEXPB8, TaEXPB10 have an important correlation with the tolerance to cold stress of winter wheat roots, which is helpful to further understand the cold-resistant mechanism of winter wheat roots.