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目的：研究参麦注射液对肝缺血再灌注损伤的保护作用。方法：将３７只成年雄性ＳＤ大鼠随机分成假手术对照组（ＳＯＣ）、缺血再灌注组（Ｉ／Ｒ）、缺血再灌注加参麦组（Ｉ／Ｒ＋ｓｈｅｎｍａｉ）。通过阻断大鼠肝门３０ｍｉｎ后再开放建立肝缺血再灌注损伤模型，在肝脏再灌注９０ｍｉｎ时测肝组织丙二醛（ＭＤＡ）、超氧化物歧化酶（ＳＯＤ）和测血ＡＬＴ、ＡＳＴ、ＬＤＨ，并取肝组织作光镜及电镜观察。结果：再灌注９０ｍｉｎ时Ｉ／Ｒ＋Ｓｈｅｎｍａｉ组的肝组织ＭＤＡ生成，ＳＯＤ消耗，血清ＡＬＴ、ＡＳＴ、ＬＤＨ升高值均少于Ｉ／Ｒ组（Ｐ＜０．０１），且Ｉ／Ｒ＋Ｓｈｅｎｍａｉ组的肝细胞显微、超微结构损害的改变较Ｉ／Ｒ组轻。结论：参麦注射液能清除肝缺血再灌注过程中产生的氧自由基，对鼠肝缺血再灌注所致肝细胞的结构和功能损伤有保护作用
Objective: To study the protective effect of Shenmai injection on liver ischemia-reperfusion injury. METHODS: Thirty-seven adult male Sprague-Dawley rats were randomly divided into sham-operated control group (SOC), ischemia-reperfusion group (I/R), and ischemia reperfusion plus Shenmai group (I/R+shenmai). Liver ischemia-reperfusion injury model was established by blocking the rat hepatic portal for 30 minutes before reopening. Liver tissue malondialdehyde (MDA), superoxide dismutase (SOD) and blood ALT, AST were measured at 90 min after reperfusion. , LDH, and liver tissue were taken for light and electron microscopy. RESULTS: At 90 min after reperfusion, MDA production, SOD consumption, serum ALT, AST, and LDH levels in the I/R+Shenmai group were all lower than those in the I/R group (P<0.01), and livers in the I/R+Shenmai group were significantly lower. Changes in cell microscopic and ultrastructural damage were lighter than those in the I/R group. Conclusion: Shenmai injection can clear the oxygen free radicals produced during the process of hepatic ischemia-reperfusion, and protect the hepatic cells from structural and functional damage caused by hepatic ischemia-reperfusion in rats.