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To explore the exogenous hydrogen peroxide- and endogenoussuperoxide-induced DNA strand breakage in human spermatozoa invitro, the spermatozoa were exposed to different concentrations ofH_2O_2 and β-NADPH for certain period of time. The resulted DNAstrand breakage was detected using single-cell gel electrophoresis.The percentage of comet cells was counted and the comet tail length
To explore the exogenous hydrogen peroxide- and endogenoussuperoxide-induced DNA strand breakage in human spermatozoa invitro, the spermatozoa were exposed to different concentrations of H 2 O 2 and β-NADPH for certain period of time. The resulting DNA strand break was detected using single-cell gel electrophoresis. The percentage of comet cells was counted and the comet tail length