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目的探讨系统性红斑狼疮(SLE)患者外周血CCR7+CD8+CD45RO+记忆性T细胞对CD4+T细胞的诱导分化作用及其与SLE发病的关系。方法流式细胞仪、实时定量RT-PCR和RNA印迹检测同系CCR7+CD8+CD45RO+T细胞和树突细胞协同刺激CD4+T细胞分泌细胞因子。结果活动期SLE患者CCR7+CD8+CD45RO+记忆性T细胞诱导CD4+T细胞表达Th2类细胞因子:白介素4的表达效率显著高于正常人对照组和非活动期SLE患者组(P<0.01),1型调节性T细胞(Tr1)源性细胞因子:白介素10和转化生长因子β的表达效率均低于正常对照组和非活动期SLE患者组(P<0.01);而活动期和非活动期SLE患者干扰素γ的表达效率显著低于正常人对照组(P<0.01)。结论活动期SLE患者外周血CCR7+中央型记忆性T细胞可与树突细胞相互作用,诱导同系CD4+T细胞向Th2分化,发挥CCR7-CD45RO+效应性记忆性T细胞的功能。
Objective To investigate the effect of CCR7 + CD8 + CD45RO + memory T cells on the differentiation of CD4 + T cells in patients with systemic lupus erythematosus (SLE) and its relationship with the pathogenesis of SLE. Methods Flow cytometry, real-time quantitative RT-PCR and Northern blotting were used to detect cytokine secretion by synergistic stimulation of CD4 + T cells with syngeneic CCR7 + CD8 + CD45RO + T cells and dendritic cells. Results The expression of interleukin-4 (IL-4) in CD4 + T cells induced by CCR7 + CD8 + CD45RO + memory T cells in active SLE patients was significantly higher than that in normal controls and inactive SLE patients (P <0.01) The expression of TRAIL-1-derived cytokines: interleukin-10 and transforming growth factor-β were lower than those in normal controls and inactive SLE patients (P <0.01). However, both active and inactive TRAIL- The expression of interferon gamma in SLE patients was significantly lower than that in normal controls (P <0.01). Conclusions CCR7 + central memory T cells in peripheral blood of patients with active SLE can interact with dendritic cells to induce the differentiation of CD4 + T cells to Th2 cells and exert the function of CCR7-CD45RO + effector memory T cells.