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乙型肝炎 (乙肝 )疫苗免疫后 ,体液和细胞免疫回忆应答的状况和特点 ,是确定是否需要加强免疫及免疫方案的关键。为此 ,在湖南省湘潭市随机采样 ,抽取 170名 1986～ 1988年出生、完成了血源乙肝疫苗全程免疫后 9～ 11年的儿童 ,用重组 (酵母 )乙肝疫苗进行 1～ 3次加强免疫 ,观察其体液免疫应答。在浙江省杭州市 ,随机抽取 2 8名血源乙肝疫苗母婴阻断免疫者、18名全程免疫儿童和 11名未免疫的健康人 ,观察他们的外周血淋巴细胞 (PBMC)对基因重组乙肝表面抗原 (HBsAg)刺激的增殖反应。结果显示 :乙肝疫苗全程免疫后 9～ 11年的儿童 ,完成 1针、2针和 3针法加强免疫后 1年内 ,乙肝病毒表面抗体 (抗 -HBs)阳性率和几何平均滴度 (GMT)均迅速升高 ,三组的GMT分别比加强免疫前提高了 6 5、9 3、2 4 2倍 ,阳性率均上升至 10 0 % ,加强免疫次数多抗体提高多 ,各组间GMT差异有非常显著的统计学意义 (P <0 0 1)。完成加强免疫后 ,3次加强免疫组抗 -HBs阳性率比 <3次组下降缓慢 ,至加强免疫后第 3年 ,抗 -HBs阳性率 1针法降为 71 2 % ,2针法降为 79 2 % ,而 3针法维持在 92 5 %。血源乙肝疫苗全程免疫者其PBMC对重组HBsAg刺激的特异性增殖反应强度和γ干扰素 (INF -γ)水平明显高于对照组 ,细胞免疫反应的强度?
The status and characteristics of humoral and cellular immune recall responses after Hepatitis B (HBV) immunization are key to determining whether an immunization and immunization program needs to be strengthened. To this end, in Xiangtan City, Hunan Province, random sampling, drawn from 1986 to 1988 was born, complete blood-borne hepatitis B vaccine after 9 to 11 years of children, with recombinant (yeast) hepatitis B vaccine for 1 to 3 times to strengthen immunity , Observe its humoral immune response. In Hangzhou, Zhejiang Province, 28 randomly selected mothers and babies with immunized hepatitis B vaccine, 18 immunized children and 11 non-immunized healthy subjects were enrolled in the study. Their peripheral blood lymphocytes (PBMC) Surface antigen (HBsAg) stimulated proliferative response. The results showed that the positive rates and the geometric mean titers (GMT) of HBsAg (anti-HBs) in children 9 to 11 years after the whole immunization of hepatitis B vaccine were within 1 year after the 1, 2 and 3 st acupuncture treatments were completed. All increased rapidly. The GMTs of the three groups increased by 65.93 and 24.2 times, respectively, and the positive rates increased to 100% and the numbers of boosted immunizations increased more. The GMT differences among the groups were Very significant statistical significance (P <0.01). After the booster immunization, the positive rate of anti-HBs in the three booster vaccination groups was slower than that in the third sub-booster group. By the third year after booster immunization, the positive rate of anti-HBs was reduced to 71.2% 79 2%, while the 3-pin method maintained at 92 5%. The specific immune response of PBMC to recombinant HBsAg stimulation and the level of IFN-γ (IFN-γ) were significantly higher than those of the control group in the whole blood-borne hepatitis B vaccine immunization.