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目的对云南省3株新型肠道病毒EV80的基因特征进行分析。方法对3株EV80病毒VP1区基因进行RT-PCR和基因测序,并在NCBI、EBI和DDBJ三大基因库搜索EV80VP1区基因序列,用MEGA5.0软件计算所得序列的核苷酸和氨基酸相似性,并做基因树进行基因特征分析。结果共检索到35株EV80的VP1区基因序列,去除序列太短不能分析的2株,对云南株和基因库中共33株进行了分析。33株病毒可分为4个基因型,原型株USA-CA67-10387单独聚成一组,为基因型A,云南株和山东株同属基因型D,为中国特有的基因型。结论虽然目前全世界新型肠道病毒EV80的分离数不多,但其地理分布较为广泛,不同国家、地区的毒株大都为不同的基因型,具有基因多样性特点。本研究对今后中国开展新型肠道病毒EV80的分子流行病学研究和传播链的发现和疫情处置提供了实验室基础。
Objective To analyze the genetic characteristics of three novel enterovirus EV80 in Yunnan Province. Methods RT-PCR and gene sequencing of VP1 gene of three EV80 viruses were carried out. The sequences of EV80 VP1 gene were searched by NCBI, EBI and DDBJ genes. The nucleotide and amino acid similarity of the EV80 VP1 gene were calculated by MEGA 5.0 software , And gene tree analysis of gene characteristics. Results A total of 35 EV80 VP1 gene sequences were retrieved and two sequences that could not be analyzed with too short sequences were deleted. A total of 33 strains in Yunnan and China were analyzed. 33 strains of viruses can be divided into four genotypes, the prototype strain USA-CA67-10387 gathered together as a group, genotype A, Yunnan and Shandong strains belong to genotype D, is China’s unique genotype. Conclusion Although the number of EV80 isolated from new enterovirus in the world is relatively small at present, its geographical distribution is quite extensive. Most of the strains in different countries and regions are of different genotypes and have the characteristics of gene diversity. This study provides a laboratory basis for the future molecular epidemiological studies on China’s new enterovirus EV80 and on the discovery and outbreak of transmission chain.