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作者从168例各类中耳炎病人中取得200份中耳渗出液进行检查,取样前2周停用抗生素。标本以革兰氏和吖啶橙染色或培养在血和巧克力琼脂的平板上,显微镜检查。按Stenfors和R(?)is(?)nen(1988)介绍的方法计算每毫升渗出液细菌数。急性中耳炎(AOM)患者中收集的标本53%(17/32)含中耳致病菌(肺炎链球菌,流感嗜血杆菌和卡他布兰汉氏菌)数量为10~6～10~8/ml,平均为10~7/ml,外耳道正常生长的细菌(类白喉菌、凝固酶阴性葡萄球菌和/或金黄色葡萄球菌)通常为10~3～10~4/ml。47%(15/32)无致病菌发现,31%(10/32)无细菌。107份粘液样渗出液标本中24份(22%)有中耳致病
The authors examined 200 cases of middle ear effusion from 168 patients with various otitis media and discontinued antibiotics two weeks before sampling. Specimens were stained with Gram and acridine orange or cultured on plates of blood and chocolate agar and examined microscopically. The number of bacteria per ml of exudate was calculated as described by Stenfors and R? Isis (?) Nen (1988). 53% (17/32) of the specimens collected from patients with acute otitis media (AOM) contained 10 ~ 6 ~ 10-8 middle ear pathogens (Streptococcus pneumoniae, Haemophilus influenzae and Branhad) / ml, an average of 10 ~ 7 / ml, the normal growth of the external auditory canal bacteria (diphtheria diphtheria coagulase-negative staphylococcus aureus and / or Staphylococcus aureus) is usually 10 ~ 3 ~ 10 ~ 4 / ml. 47% (15/32) of non-pathogenic bacteria found, 31% (10/32) of bacteria-free. Twenty-four (22%) of the 107 samples of mucoid exudate had middle ear disease