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目的:建立八味沉香胶囊中槲皮素的含量测定方法。方法:高效液相色谱法。色谱柱为Phe-nomen C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-1%冰醋酸水溶液(37∶63),检测波长为371 nm。结果:进样量在0.047~0.236μg范围内线性良好,A=89 003X-6 199.7,r=0.999 8,加样回收率为98.8%(n=6),RSD为1.23%。结论:本方法操作简单,结果准确,可用于八味沉香胶囊的质量控制。
Objective: To establish a method for the determination of quercetin in Bawei Shenxiang capsule. Methods: High performance liquid chromatography. The chromatographic column was Phe-nomen C18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol-1% glacial acetic acid (37:63) and the detection wavelength was 371 nm. Results: The linearity was good in the range of 0.047-0.236 μg, with A = 89 003X-6 199.7 and r = 0.999 8. The recovery rate was 98.8% (n = 6) and the RSD was 1.23%. Conclusion: The method is simple and accurate. It can be used for the quality control of Bawei Shenxiang capsule.