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本试验以亚麻幼苗的下胚轴为外植体 ,利用抗除草剂 Basta的目的基因和 GUSINT基因 ,采用农杆菌介导法进行了亚麻转基因过程中植株再生及生根适宜培养基的筛选试验。经试验得出 :在附加少量 BA和 NAA的 Ms培养基上愈伤组织的再分化率可达31.8%～ 40 .2 %。经 GUS基因检测在脱菌后 3天亚麻下胚轴初步形成的愈伤组织的转化率可达 72 .2 %;四周后的转化率为 2 5 %;再生植株的转化率为 2 0 %。在 1/2 Ms培养基中附加 0 .0 0 1mg/L NAA可使再生植株的生根率达到 87.4%。通过试验初步建立起了根瘤农杆菌介导法亚麻转基因系统。
In this experiment, the hypocotyls of flax seedlings were used as explants. By using Agrobacterium tumefaciens-mediated method, the target gene of herbicide Basta and GUSINT gene were used to screen the plant regeneration and rooting suitable medium of flax transgenics. The results showed that the rate of redifferentiation of callus on Ms medium supplemented with a small amount of BA and NAA was 31.8% -40.2%. The callus induction rate of the hypocotyls of flax was up to 72.2% after 3 days sterilized by GUS gene detection; the transformation rate was 25% after 4 weeks; the conversion of regenerated plants was 20%. Addition of 0.010 mg / L NAA in 1/2 Ms medium resulted in 87.4% of rooting rate of regenerated plants. Through experiments, we initially established Agrobacterium tumefaciens-mediated flax transgenic system.