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目的:比较乌司他丁的效价测定方法,提高检测速度和质量。方法:分别用酶标仪和紫外分光光度计进行乌司他丁的效价测定。结果用紫外法和酶标法测定的结果基本一致。结论:采用酶标仪测定乌司他丁的效价可以节省试剂用量和减少样品量,提高检验的工作效率。
OBJECTIVE: To compare the potency determination of ulinastatin and improve the detection speed and quality. Methods: The titer of ulinastatin was determined by microplate reader and UV spectrophotometer respectively. Results The results of UV and ELISA were basically the same. Conclusion: The determination of ulinastatin titer by microplate reader can save reagents and reduce the sample size, and improve the efficiency of the test.