论文部分内容阅读
目的建立田银通脉颗粒中三七总皂苷中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的含量测定方法。方法采用高效液相色谱法测定田银通脉颗粒中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的含量。色谱柱为Diamon-silTMC18(250 mm×4.6 mm,5μm);流动相:乙腈(A)-0.05%磷酸水(B)梯度洗脱(0～8 min,20%A;8～40 min20%～30%A;40～60 min,30%～45%A);流速为1.0 ml/min;柱温为室温;检测波长:203 nm。结果三七皂苷R1在0.212～3.392μg范围内线性关系良好,r=0.999 6,平均回收率为100.21%,RSD=1.71%(n=9);人参皂苷Rg1在0.832～13.312μg范围内线性关系良好,r=0.999 5,平均回收率为100.24%,RSD=1.62%(n=9),人参皂苷Rb1在0.816～13.056μg范围内线性关系良好,r=0.999 7,平均回收率为99.66%,RSD=1.59%(n=9)。结论所建立的高效液相色谱法测定田银通脉颗粒中的三七皂苷R1、人参皂苷Rg、人参皂苷Rb1含量的方法准确、可靠,专属性强,可有效控制田银通脉颗粒的质量。
Objective To establish a method for the determination of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Pan Yin Tong Mai Granules. Methods The contents of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Tianyin Tongmai Granules were determined by high performance liquid chromatography. The chromatographic column was Diamon-silTMC18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of a gradient of acetonitrile (A) -0.05% phosphoric acid (B) (0-8 min, 20% A; 30% A; 40-60 min, 30% -45% A); the flow rate was 1.0 ml / min; the column temperature was room temperature; the detection wavelength was 203 nm. Results The notoginsenoside R1 had a good linear relationship in the range of 0.212 ~ 3.392μg, r = 0.999 6, the average recovery was 100.21% and the RSD was 1.71% (n = 9). The linear range of ginsenoside Rg1 was 0.832 ~ 13.312μg The average recovery was 100.24%, RSD = 1.62% (n = 9). The linear range of ginsenoside Rb1 was 0.816 ~ 13.056μg with r = 0.999 7 and the average recovery was 99.66% RSD = 1.59% (n = 9). Conclusion The established HPLC method for the determination of notoginsenoside R1, ginsenoside Rg and ginsenoside Rb1 in Tianyin Tongmai Granules is accurate, reliable and specific and can effectively control the quality of Tianyin Tongmai Granules.