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目的研究人鼻病毒(HRV)感染对HMC-1肥大细胞先天免疫应答功能的调控作用。方法实时定量PCR检测HRV在肥大细胞上的复制水平,流式细胞术检测肥大细胞的凋亡,ELISA检测肿瘤坏死因子α(TNF-α)、α干扰素(IFN-α)、白细胞介素6(IL-6)和IL-8水平。结果 HRV感染HMC-1肥大细胞后,病毒的拷贝数明显升高,凋亡细胞明显增加,TNF-α、IFN-α、IL-6和IL-8释放增加。结论 HRV感染促进肥大细胞凋亡、增加炎性细胞因子释放。
Objective To study the regulatory effect of human rhinovirus (HRV) infection on the innate immune response of HMC-1 mast cells. Methods Real-time quantitative PCR was used to detect the level of HRV replication in mast cells. Flow cytometry was used to detect the apoptosis of mast cells. The levels of tumor necrosis factor alpha (TNF-α), interferon-alpha (IFN-α), interleukin- (IL-6) and IL-8 levels. Results The infection of HMC-1 with HRV significantly increased the copy number of the virus, increased the apoptotic cells and increased the release of TNF-α, IFN-α, IL-6 and IL-8. Conclusion HRV infection can promote the apoptosis of mast cells and increase the release of inflammatory cytokines.