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目的:观察H102在细胞水平上对人神经母细胞瘤株SY5Y生长的影响,寻找最佳药物浓度及对Aβ42所致神经元毒性的保护作用。方法:将不同浓度的H102作用于人神经母细胞瘤株SY5Y,利用噻唑蓝(MTT)法测定细胞的存活率,制定浓度-药效曲线得出最佳药物浓度;将人神经母细胞瘤株SY5Y细胞接种后分为对照组、Aβ42损伤模型组及Aβ42损伤加H102保护组,观察各组的MTT代谢率、乳酸脱氢酶(LDH)漏出率、细胞形态。结果:H102在10～160μmol/L浓度范围内均可增加SY5Y细胞的存活率(P<0.01),20μmol/L是增加细胞活性的最适浓度;与对照组相比,Aβ42损伤模型组MTT代谢率下降(P<0.05)、LDH漏出率增高(P<0.01),细胞突起损伤、死细胞增多,加入H102保护后可使上述指标恢复或接近正常。结论:H102具有神经保护作用,可减轻由Aβ42所致的神经元毒性。
OBJECTIVE: To observe the effect of H102 on the growth of human neuroblastoma SY5Y at the cellular level in order to find out the optimal drug concentration and the protective effect on Aβ42 induced neuronal toxicity. Methods: Different concentration of H102 was applied to human neuroblastoma cell line SY5Y. The cell viability was determined by MTT assay and the best concentration of drug was obtained. The concentration of human neuroblastoma SY5Y cells were divided into control group, Aβ42 injury group and Aβ42 injury plus H102 protection group. MTT metabolic rate, lactate dehydrogenase (LDH) leakage rate and cell morphology were observed in each group. Results: H102 could increase the survival rate of SY5Y cells (P <0.01) in the concentration range of 10 ~ 160μmol / L, and the optimal concentration of 20μmol / L could increase the cell viability. Compared with the control group, MTT metabolism (P <0.05). The leakage rate of LDH increased (P <0.01), and the cell damage and dead cells increased. After the protection of H102, the above indexes were recovered or nearly normal. Conclusion: H102 has neuroprotective effect and can reduce neuronal toxicity induced by Aβ42.