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Nickel ferrite(NiFe_2O_4) nanoparticles have been dispersed in chitosan solution in order to fab ricate nanocomposite films.Horseradish peroxidase(HRP) has been immobilized onto this chitosan NiFe_2O_4 nanocomposite film via physical adsorption.The size of the NiFe_2O_4 nanoparticles has been estimated us ing X ray diffraction pattern and scanning electron microscopy(SEM) to be 40±9 nm.The chitosan NiFe_2O_4 nanocomposite film and HRP/chitosan NiFe_2O_4 bioelectrode have been characterized using SEM technique.The HRP/chitosan NiFe_2O_4 nanocomposite bioelectrode has a response time of 4 s,linearity as 0.3 to 12 m M of H2O2,sensitivity as 22 n A/m M.The effects of p H and the temperature of the immobilized HRP electrode have also been studied.
Nickel ferrite (NiFe 2 O 4) nanoparticles have been dispersed in chitosan solution in order to fab ricate nanocomposite films. Horseradish peroxidase (HRP) has been immobilized onto this chitosan NiFe 2 O 4 nanocomposite film via physical adsorption. The size of the NiFe 2 O 4 nanoparticles has been estimated us ing X ray diffraction pattern and scanning electron microscopy (SEM) to be 40 ± 9 nm. The chitosan NiFe 2 O 4 nanocomposite film and HRP / chitosan NiFe 2 O 4 bioelectrode have been characterized using SEM technique. The HRP / chitosan NiFe 2 O 4 nanocomposite bioelectrode has a response time of 4 s, linearity as 0.3 to 12 m M of H2O2, sensitivity as 22 n A / m M. The effects of p H and the temperature of the immobilized HRP electrode have also been studied.