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目的:建立灯心草中绿原酸、隐绿原酸、咖啡酸和木犀草素UPLC含量测定方法。方法:采用AgilentPoroshell 120 EC-C18色谱柱(100 mm×3.0 mm,2.7μm),以乙腈-0.1%的甲酸水溶液为流动相,梯度洗脱,流速0.4 mL/min,检测波长325和350 nm,柱温30℃。结果:绿原酸、隐绿原酸、咖啡酸和木犀草素分别在0.65～64.96μg/mL(r1=0.9997)、0.16～16.32μg/mL(r2=0.9999)、0.33～32.64μg/mL(r3=0.9999)和0.41～40.60μg/mL(r4=0.9998)内线性关系良好;平均加样回收率(n=6)在95.8%～102.7%,RSD均小于4.7%。结论:该方法快速、准确、重现性好,可用于灯心草中绿原酸、隐绿原酸、咖啡酸和木犀草素的含量测定。
Objective: To establish a method for the determination of chlorogenic acid, cryptocholic acid, caffeic acid and luteolin UPLC in Radix Hedysari. Methods: The mobile phase was acetonitrile-0.1% formic acid aqueous solution with a gradient of 0.4 mL / min on an Agilent Poroshell 120 EC-C18 column (100 mm × 3.0 mm, 2.7 μm) Column temperature 30 ℃. RESULTS: The concentrations of chlorogenic acid, cryptocholic acid, caffeic acid and luteolin in the range of 0.65-64.96μg / mL (r1 = 0.9997), 0.16-16.32μg / mL (r2 = 0.9999) and 0.33-32.64μg / mL r3 = 0.9999) and 0.41 ~ 40.60μg / mL (r4 = 0.9998). The average recoveries were 95.8% -102.7% with RSD less than 4.7%. Conclusion: The method is rapid, accurate and reproducible. It can be used for the determination of chlorogenic acid, cryptocholic acid, caffeic acid and luteolin in JXH.