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目的观察磷酸肌酸钠及丹参酮磺酸钠酮ⅡA磺酸钠对阿霉素心肌病大鼠心室肌细胞单通道钙电流的影响。方法 40只wister雄性大鼠分为磷酸肌酸钠+阿霉素组干预组(n=10),丹参酮ⅡA磺酸钠+阿霉素干预组(n=10),阿霉素组(n=10)和正常对照组(n=10)。观察大鼠的一般情况,14d处死取心脏标本,Langendorff离体心脏逆向灌流法分离大鼠心室肌细胞,随机选取心室肌细胞分为对照组、模型组、模型+磷酸肌酸钠组和模型+丹参酮ⅡA磺酸钠组。应用细胞贴附式方式记录心室肌细胞钙电流,分析对钙单通道电流的影响。结果磷酸肌酸钠和丹参酮ⅡA磺酸钠可使模型组心室肌细胞钙通道开放时间,开放概率明显降低(p<0.05),关闭时间明显增高(p<0.05)。结论丹参酮ⅡA磺酸钠和磷酸肌酸钠通过降低心室肌细胞钙通道开放时间、增加关闭时间、降低开放概率抑制心室肌细胞钙电流。
Objective To investigate the effects of sodium creatinine and sodium tanshinone sulfonate Ⅱ A on the single-channel calcium currents in ventricular myocytes of adriamycin-induced cardiomyopathy rats. Methods Forty wister male rats were randomly divided into sodium phosphate + doxorubicin group (n = 10), tanshinone Ⅱ A sulfonate + doxorubicin group (n = 10), doxorubicin group (n = 10) and normal control group (n = 10). The rats were sacrificed and hearts were sacrificed on the 14th day. Langendorff hearts were isolated by reverse perfusion, and ventricular myocytes were randomly divided into control group, model group, model group + sodium creatine phosphate and model + Tanshinone Ⅱ A sodium sulfonate group. Calcium current of ventricular myocytes was recorded by cell attachment method and the effect on calcium single channel current was analyzed. Results Sodium creatine phosphate and sodium tanshinone ⅡA could significantly decrease the open time and the open probability of calcium channels in ventricular myocytes in model group (p <0.05), and significantly decrease the time of closure (p <0.05). Conclusion Sodium tanshinone Ⅱ A sulfonate and sodium creatine phosphate can decrease the calcium channel opening time of ventricular myocytes, increase the closing time and decrease the open probability of inhibiting calcium current in ventricular myocytes.