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目的探讨血管内皮生长因子(VEGF)、可溶性细胞间黏附分子-1(sICAM-1)蛋白在结直肠癌患者血清中的表达水平及临床意义。方法分别选择66例结直肠癌患者(结直肠癌组)和40例健康者(对照组),采用酶联免疫吸附测定法(ELISA)测定两组患者血清VEGF、sICAM-1蛋白表达水平,分析VEGF、sICAM-1蛋白表达水平与结直肠癌患者肿瘤生长部位、TNM分期、肿瘤浸润深度及淋巴结转移的关系。结果结直肠癌组血清VEGF、sICAM-1蛋白表达水平均显著高于对照组(P均<0.01);结肠癌患者血清sICAM-1蛋白表达水平显著高于直肠癌患者(P<0.01);Ⅳ期患者血清VEGF、sICAM-1蛋白表达水平明显高于Ⅰ、Ⅱ、Ⅲ期患者(P均<0.01);肿瘤浸润肠壁全层的患者血清VEGF水平明显高于未浸润肠壁全层的患者(P<0.01);有局部淋巴结转移的患者血清VEGF水平明显高于无局部淋巴结转移的患者(P<0.01)。结论血清VEGF、sICAM-1蛋白表达水平可能是结直肠癌有价值的辅助诊断及判断复发转移的指标。
Objective To investigate the expression of vascular endothelial growth factor (VEGF) and soluble intercellular adhesion molecule-1 (sICAM-1) in serum of patients with colorectal cancer and its clinical significance. Methods Sixty-six patients with colorectal cancer (colorectal cancer group) and 40 healthy controls (control group) were enrolled in this study. Serum levels of VEGF and sICAM-1 were measured by enzyme-linked immunosorbent assay (ELISA) VEGF, sICAM-1 protein expression in colorectal cancer patients with tumor growth sites, TNM staging, tumor invasion depth and lymph node metastasis. Results The levels of VEGF and sICAM-1 protein in colorectal cancer were significantly higher than those in control group (all P <0.01). The expression of sICAM-1 protein in colorectal cancer was significantly higher than that in rectal cancer (P <0.01) Serum levels of VEGF and sICAM-1 were significantly higher in patients with stage Ⅰ, Ⅱ and Ⅲ (all P <0.01). Serum levels of VEGF in patients with full-thickness tumor infiltration were significantly higher than those in non-infiltrated (P <0.01). Serum VEGF levels in patients with local lymph node metastasis were significantly higher than those without lymph node metastasis (P <0.01). Conclusions The serum levels of VEGF and sICAM-1 may be valuable diagnostic and prognostic indicators for colorectal cancer.