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对FⅧ cDNA进行分子改建,并分别克隆到表达载体中,在多种哺乳动物细胞表达体系（CHO、BHK等）成功地获得了表达。结果证实:基因的改建可以有效的提高FⅧ在细胞中的表达。FⅧB结构域的缺失比全长FⅧ的表达量提高了3倍左右,而重链和轻链的共表达及vMF与FⅧ的共同转染使得其表达量进一步提高。与CHO表达系统相比较,FⅧ在BHK表达系统中的表达量提高了近10倍。
The F Ⅷ cDNA was molecularly modified and cloned into the expression vector respectively. Expression was successfully obtained in various mammalian cell expression systems (CHO, BHK, etc.). The results confirmed that the genetic modification can effectively improve the FⅧ expression in cells. FⅧB domain deletion than the full-length F Ⅷ expression increased about 3 times, and heavy chain and light chain co-expression and vMF and F Ⅷ co-transfected the expression of further increased. Compared with the CHO expression system, FⅧ expression in BHK expression system increased by nearly 10 times.