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目的:观察油酸致大鼠ARDS过程中肾组织TNF-α、IL-10在基因及蛋白水平表达的变化。方法:采用油酸(Oleic Acid,OA)法将16只Wister大鼠复制ARDS模型,随机分为对照组和实验组(油酸6 h组),测定肾功能,解剖取肺、肾脏同时测定肺湿/干(W/D)。应用逆转录-聚合酶链反应法(RT-PCR)检测大鼠肾组织TNF-α、IL-10基因的表达;采用放射免疫法检测大鼠血、肾脏组织TNF-α及IL-10蛋白的表达;光镜观察肾组织病理学改变。结果:实验组较对照组肾功能检测值增高;TNF-α、IL-10 mRNA的表达在血清及肾脏增强;TNF-α、IL-10蛋白的表达在血浆及肾脏增强;病理切片肾组织有结构改变。结论:TNF-α、IL-10可能参与ARDS致肾功能障碍的发病机理。
Objective: To observe the changes of gene and protein expression of TNF-α and IL-10 in renal tissue induced by oleic acid in ARDS rats. Methods: Sixteen Wister rats were randomly divided into control group and experimental group (oleic acid 6 h group) by Oleic Acid (OA) method. Renal function was measured. Pulmonary and renal were dissected to determine lung Wet / dry (W / D). The expression of TNF-α and IL-10 gene in renal tissue was detected by reverse transcription-polymerase chain reaction (RT-PCR). The levels of TNF-α and IL-10 in blood and kidney tissue were detected by radioimmunoassay The pathological changes of renal tissue were observed by light microscope. Results: Compared with the control group, the renal function of the experimental group increased, the expression of TNF-α and IL-10 mRNA increased in the serum and kidney, the expression of TNF-α and IL-10 in the plasma and kidney increased, Structural change. Conclusion: TNF-α and IL-10 may be involved in the pathogenesis of ARDS-induced renal dysfunction.