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采用固体平板和液体摇瓶实验,以自玉米根际分离获得的10株优良溶磷菌为材料,进一步筛选出4株兼具较好利用Ga3(PO4)2,AlPO4,FePO4和卵磷脂的溶磷菌株(P1,P17,P35,P21).4株溶磷菌对不同难溶磷的溶解能力从大到小依次为Ga3(PO4)2、混合难溶磷>AlPO4、FePO4>卵磷脂.其中,菌株P21对混合难溶磷、AlPO4、FePO4以及卵磷脂的溶磷量(全磷、有效磷)均为最大,该菌株在上述各难溶磷培养液中的全磷分别为128.84,30.23,27.20和6.79μg/mL,有效磷分别为90.27,25.03,21.47和6.53μg/mL,对Ca3(PO4)2也能较好地溶解(全磷85.07μg/mL、有效磷56.68μg/mL),表明在室内培养条件下,菌株P21对多种难溶磷的兼溶能力更为突出.盆栽实验表明,与CK相比,4株溶磷菌均能显著提高玉米幼苗干物质量、可溶性糖和可溶性蛋白质量分数及根系活力;可能受多种环境因素影响,菌株P21对幼苗的促生效应不及P17,P35菌株突出,表现出溶磷菌实际应用的复杂性.
Using solid plate and liquid shake flask experiments, 10 strains of good phosphate-solubilizing bacteria isolated from the rhizosphere of maize were used as materials to further screen out 4 strains which have better utilization of Ga3 (PO4) 2, AlPO4, FePO4 and lecithin (P1, P17, P35, P21) .Among four strains of phosphate-solubilizing bacteria, the solubilities of different refractory phosphorous were Ga3 (PO4) 2 and mixed with insoluble phosphorus> AlPO4, FePO4> lecithin The total phosphorus (total phosphorus and available phosphorus) of strain P21 was the highest for the mixed insoluble phosphorus, AlPO4, FePO4 and lecithin, and the total phosphorus of the strain in each of the above-mentioned refractory phosphorus culture solutions was 128.84 and 30.23, 27.20 μg / mL and 6.79 μg / mL respectively, and available phosphorus were 90.27, 25.03, 21.47 and 6.53 μg / mL, respectively, which also could dissolve Ca3 (PO4) 2 well (total phosphorus 85.07μg / mL, available phosphorus 56.68μg / mL) The results showed that under the indoor culture conditions, the ability of strain P21 to dissolve soluble phosphorus was more prominent.The pot experiments showed that compared with CK, all four phosphate-solubilizing bacteria could significantly increase the dry matter content, soluble sugar and soluble Protein content and root activity may be affected by a variety of environmental factors, P21 strain on seedling growth promoting effect is less than P17, P35 strains prominent, showing phosphate-solubilizing bacteria The complexity of the international application.