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目的探讨四黄前列片对小鼠前列腺增生模型的影响。方法选用NIH系雄性小鼠,依文献方法,行前列腺腹叶内植入尿生殖窦组织,制备BPH动物模型,给药30D后处死动物,取前列腺组织,称重测量体积,组织切片观察前列腺腺腔面积和腺上皮高度,用免疫组化法研究实验各组前列腺组织中PCNA抗原和CASPASE-3蛋白的表达。结果四黄前列片可缩小前列腺体积,降低腺腔的面积和腺上皮高度,并能抑制前列腺组织中PCNA的表达,增加CASPASE-3的表达。结论四黄前列片能抑制模型小鼠前列腺增生,其机理可能与抑制前列腺细胞的增殖和诱导凋亡有关。
Objective To investigate the effect of Sihuangqianlie tablet on BPH model in mice. METHODS: Male NIH mice were selected and the urogenital sinus tissue was implanted into the prostate in the ventral prostate gland according to the literature method. The animal model of BPH was prepared. The animals were sacrificed 30 days after the administration. Prostate tissues were taken, the volume was measured, and the prostate glands were sectioned. The luminal area and height of glandular epithelium were used to study the expression of PCNA antigen and CASPASE-3 protein in prostate tissue of each experimental group by immunohistochemistry. Results Sihuangqianlie tablet can reduce the volume of prostate, reduce the area of glandular cavity and the height of glandular epithelium, and can inhibit the expression of PCNA in prostate tissue and increase the expression of CASPASE-3. Conclusion Sihuangqianlie tablet can inhibit the proliferation of benign prostatic hyperplasia in model mice. The mechanism may be related to the inhibition of prostatic cell proliferation and induction of apoptosis.