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目的 :检测红细胞膜跨膜蛋白质———血型糖蛋白Ａ(ＧＰＡ)对酵母细胞是否有毒性及其能否激活检测基因 ,以进一步揭示ＧＰＡ与带Ⅲ蛋白的相互关系。材料与方法 :培养红白血病细胞 (Ｋ562 )直到细胞总数达到1 0 9以上 ,收获细胞 ,用于粗提核内容物的制备。收集增殖状态Ｋ562细胞
OBJECTIVE: To detect whether the transmembrane protein of erythrocyte membrane-glycoprotein A (GPA) is toxic to yeast cells and whether it can activate the detection of GPA to further reveal the relationship between GPA and Bel-3 protein. MATERIALS AND METHODS: Erythroleukemia cells (K562) were cultured until the total number of cells reached more than 109, and cells were harvested for the preparation of the crude nuclear contents. K562 cells were collected for proliferation status