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目的研究组织因子途径抑制物2(TFPI-2)对胰腺癌血管生成的影响,探讨其抑制胰腺癌生长及侵袭、转移的机制。方法建立裸鼠角膜微囊移植模型,将3组细胞Panc-1-TFPI-2、Panc-1-P和Panc-1-V分别接种裸鼠角膜微囊,观察角膜新生血管的形成;再将上述3组细胞接种于裸鼠皮下,观察裸鼠皮下肿瘤生长及转移情况,并采用抗CD34抗体进行血管免疫组织化学染色检测皮下肿瘤的微血管密度(MVD)。Panc-1-TFPI-2组为实验组,Panc-1-P和Panc-1-V组作为对照组。结果实验组角膜新生血管积分比对照组明显减少(P<0.05),实验组和对照组裸鼠皮下均成瘤,实验组肿瘤体积(438.0±69.8)mm~3,对照组分别为(852.0±102.9)mm~3和(831.0±78.1) mm~3(P<0.05);同对照组比较,实验组未见明显远处转移,其肿瘤微血管密度(9.68±1.12),对照组分别为(18.69±2.51)和(20.32±2.08),差异有统计学意义(P<0.05)。结论组织因子途径抑制物2能抑制肿瘤血管的形成,抑制胰腺癌生长。
Objective To investigate the effect of tissue factor pathway inhibitor 2 (TFPI-2) on angiogenesis in pancreatic cancer and to explore its mechanism of inhibiting the growth, invasion and metastasis of pancreatic cancer. Methods The nude mice model of corneal microencapsulation was established. Three groups of cells, Panc-1-TFPI-2, Panc-1-P and Panc-1-V were inoculated nude mice corneal microcapsules respectively to observe the formation of corneal neovascularization The above three groups of cells were inoculated subcutaneously in nude mice to observe the growth and metastasis of subcutaneous tumor in nude mice. Anti-CD34 antibody was used to detect the microvessel density (MVD) of subcutaneous tumor by immunohistochemical staining. Panc-1-TFPI-2 group as experimental group, Panc-1-P and Panc-1-V group as control group. Results The corneal neovascularization score of the experimental group was significantly lower than that of the control group (P <0.05). The tumor volume of the experimental group and the control group was 438.0 ± 69.8 mm ~ 3, while the control group was (852.0 ± 102.9) mm ~ 3 and (831.0 ± 78.1) mm ~ 3, respectively (P <0.05). Compared with the control group, no significant distant metastasis was observed in the experimental group. The microvessel density (9.68 ± 1.12) ± 2.51) and (20.32 ± 2.08) respectively, the difference was statistically significant (P <0.05). Conclusion Tissue factor pathway inhibitor 2 can inhibit the formation of tumor blood vessels and inhibit the growth of pancreatic cancer.