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目的探讨抗癌活性肽(anti-cancer bioactive peptide,ACBP)肝肽和科脾肽对人鼻咽癌细胞株CNE体外增殖抑制作用及细胞周期的影响。方法将人鼻咽癌CNE细胞与不同浓度的ACBP进行体外培养,采用四甲基偶氮唑蓝法测定不同浓度的ACBP对CNE细胞的生长抑制率;光镜下观察形态学变化;流式细胞仪分析细胞周期与凋亡率。结果质量浓度5．0-20．0μg／ml的ACBP均能抑制CNE细胞的生长,且随浓度及作用时间延长抑制率上升,20．0μg／ml ACBP作用72 h后,肝肽组的抑制率为63．0％,科脾肽组抑制率为46．7％。光镜下可观察到ACBP作用后的细胞凋亡现象。流式细胞仪结果显示:5．0μg/ml肝肽和科脾肽作用CNE细胞24 h的早期凋亡率较高,分别为(11．8±0．3)％和(8．1±0．2)％,分别与对照组比较,差异均有统计学意义(t值分别为42．535和47．300, P值均为0．000);20．0μg／ml的肝肽和科脾肽作用CNE细胞,随作用时间延长S期细胞构成比有明显上升。结论肝肽和科脾肽对CNE细胞均有抑制增殖的作用,其抗鼻咽癌细胞增殖作用机制可能为诱导肿瘤细胞凋亡,调控肿瘤细胞周期。
Objective To investigate the effects of anti-cancer bioactive peptide (ACBP) hepatic peptide and co-peptide on proliferation inhibition and cell cycle of human nasopharyngeal carcinoma cell line CNE in vitro. Methods CNE cells of human nasopharyngeal carcinoma and ACBP of different concentrations were cultured in vitro. The growth inhibition rates of CNE cells with different concentrations of ACBP were measured by MTT method. Morphological changes were observed under light microscope. Flow cytometry Analysis of cell cycle and apoptosis rate. Results ACBP at the concentration of 5.0-20.0μg / ml could inhibit the growth of CNE cells. The inhibitory rate of CNE cells was increased with the concentration and duration of action. After 72 h treatment with 20.0μg / ml ACBP, the inhibition rate of hepatic peptide group Was 63.0%, and the inhibition rate of Kepi peptide group was 46.7%. Under light microscope, the phenomenon of apoptosis after ACBP was observed. The results of flow cytometry showed that the apoptotic rate of CNE cells treated with 5.0μg / ml hepatic peptide and Comacyl peptide for 24 hours were (11.8 ± 0.3)% and (8.1 ± 0) .2)%, respectively, compared with the control group, the differences were statistically significant (t values were 42.535 and 47.300, P values were 0.000); 20.0μg / ml of the peptide and the spleen Peptide CNE cells, with the extension of the role of S phase cells increased significantly. Conclusions Both Hepatic peptide and Coptidis Peptide can inhibit the proliferation of CNE cells. The mechanism of the anti-proliferation activity of CNE cells may be to induce tumor cell apoptosis and regulate the cell cycle of tumor cells.