用二烯酮结构代替不稳定的二烯二酮结构合成了3个结构稳定的单羰基姜黄素衍生物1,5-双(4-羟基)-1,4-戊二烯-3-酮(1a)、2,5-双(4-羟基-苯亚甲基)环戊酮(1b)和2,6-双(4-羟基-苯亚甲基)环己酮(1c)。运用荧光光谱法研究了化合物与酪氨酸酶间的相互作用。实验结果表明,酪氨酸酶可致使合成化合物的荧光强度猝灭。化合物1a结合常数KA=104.8L.mol-1,结合位点n=0.95;1b的结合常数KA=106.4L.mol-1,结合位点n=1.21;化合物1c的结合常数KA=105.1L.mol-1,结合位点n=1.05。 Three structurally stable monocarbonylcurcumin derivatives, 1,5-bis (4-hydroxy) -1,4-pentadiene-3-one were synthesized using the dienone structure instead of the labile dien-dione structure 1a), 2,5-bis (4-hydroxy-benzylidene) cyclopentanone (1b) and 2,6-bis (4-hydroxy-benzylidene) cyclohexanone (1c). The interaction between the compound and tyrosinase was studied by fluorescence spectroscopy. The experimental results show that tyrosinase can cause the fluorescence intensity of synthetic compounds to quench. The binding constant of compound 1a was KA = 104.8L.mol-1, the binding site n = 0.95; the binding constant of 1b was KA = 106.4L.mol-1, the binding site n = 1.21; the binding constant KA of compound 1c was 105.1L. mol-1, binding site n = 1.05.