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目的:建立测定香柑内酯(berg)血浆药物浓度的液相色谱-电喷雾离子化-质谱(LC-ESI-MS)联用的分析方法,并探讨其在大鼠体内的药动学。方法:采用Hypersil ODS柱(4.6 mm×250 mm,5 mm),流动相甲醇-水(77.5∶2.5),流速0.8mL.min-1,柱温30℃,检测波长300 nm。结果:Berg在8.12～162.4μg.L-1(r=0.999 9)线性关系良好。方法学考察均符合要求。日内、日间变异系数(RSD)均<10%,精密度和准确度等均符合生物样品分析的要求。结论:该法准确、灵敏、特异,适用于berg的体内定量分析,berg在大鼠体内药动学过程属于一级吸收二室模型。
OBJECTIVE: To establish a liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) combined with plasma concentration determination of bergamot and investigate its pharmacokinetics in rats. Methods: Hypersil ODS column (4.6 mm × 250 mm, 5 mm) was used. The mobile phase was methanol-water (77.5: 2.5) and the flow rate was 0.8 mL.min-1. Results: Berg had a good linear relationship between 8.12 and 162.4 μg.L-1 (r = 0.999 9). Methodological studies are in line with the requirements. Day and day, the coefficient of variation (RSD) were <10%, precision and accuracy are in line with biological sample analysis requirements. Conclusion: The method is accurate, sensitive and specific and suitable for in vivo quantitative analysis of berg. The pharmacokinetics of berg belongs to the first-order absorption two-compartment model in rats.