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目的:研究去甲乌药碱(higenamine)与6-姜酚(6-gingerol)单体及其配伍在正常心肌细胞的正性肌力作用。方法:急性分离成年SD大鼠左心室肌细胞,分别使用不同单体和单体间不同浓度的相互配伍作用于心肌细胞,使用Ion Optix单细胞收缩与离子测量系统检测细胞的最大收缩舒张速率(±dp/dt)、收缩幅度(ph/bl)、50%收缩舒张时间常数(T50S,T50D)的变化。结果:0.03～1μmol.L-1去甲乌药碱能够浓度依赖性增强心肌收缩力和最大收缩舒张速率,起效浓度为0.1μmol.L-1,1μmol.L-1能到最大效应,其使收缩幅度增加为234%,最大收缩速率增加为219%,最大舒张速率增加为272%,其对50%收缩舒张常数无影响,EC50约为0.205μmol.L-1,>1μmol.L-1即产生心肌毒性作用。3～30μmol.L-16-姜酚对心肌细胞收缩功能无显著影响,大于30μmol.L-1使心肌细胞产生不节律收缩。相互配伍不能增强去甲乌药碱的强心作用。结论:去甲乌药碱具有强心作用,与6-姜酚配伍不能显著增强去甲乌药碱的强心作用。
Objective: To study the positive inotropic effect of higenamine and 6-gingerol monomer and their compatibility in normal cardiomyocytes. Methods: Acute myocardial isolation of left ventricular myocytes from adult SD rats was performed using different concentrations of different monomers and monomers, respectively. The optimal contractile and diastolic velocities were measured using Ion Optix single cell contractility and ionometry ± dp / dt), systolic amplitude (ph / bl) and 50% systolic and diastolic time constants (T50S, T50D). Results: The effects of 0.03 ~ 1μmol.L-1 norfloxacin on myocardial contractility and maximal systolic and diastolic velocities were enhanced in a concentration-dependent manner. The maximal effective rate was 0.1μmol.L-1,1μmol.L-1 The systolic amplitude increased to 234%, the maximum systolic rate increased to 219% and the maximum diastolic rate increased to 272%, which had no effect on the 50% systolic and diastolic parameters with an EC50 of about 0.205μmol.L-1 and> 1μmol.L-1 That is to produce myocardial toxicity. 3 ~ 30μmol.L-16-gingerol had no significant effect on the contractile function of cardiomyocytes, while the greater than 30μmol.L-1 produced non-circulatory cardiomyocytes contractile. Mutual compatibility does not enhance the cardiotonic effect of demeton-alanya. CONCLUSION: Ametropine has a cardiotonic effect. Compatibility with 6-Gingerol can not significantly enhance the cardiotonic effect of norepinephrine.