各种骨髓瘤患者的M蛋白均有个体基因型(Idiotype)抗原决定簇,可产生抗独特型抗体(Anti-idjotype antibody)。一个病人的体内可产生两种以上的M蛋白,且常有相同的独特型。有人报道用吸收过的抗Id抗体检测IgA骨髓瘤独特型B淋巴细胞。但他们是按照Abdou法。采用患者血清制备抗体操作烦琐复杂。我们首次利用两例双克隆骨髓瘤患者的肾脏受损而排出非选择性蛋白尿的特点,从尿巾收集骨髓瘤蛋白,免疫家兔制备抗独特型抗休成功,报道如下。 M proteins in various myeloma patients have an individual idiotype epitope that produces an anti-idjotype antibody. A patient’s body produces more than two kinds of M protein, and often the same idiotype. It has been reported that IgA myeloma idiopathic B lymphocytes are detected with an absorbed anti-Id antibody. But they are in accordance with the Abdou method. Antibody preparation using patient serum is cumbersome and complex. We first used two cases of patients with dual-clonal myeloma kidney damage and non-selective excretion of proteinuria characteristics, collecting myeloma protein from the diaper, immune rabbit preparation anti-idiotypic success, reported as follows.