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目的 探讨己酮可可碱对肺缺血再灌注损伤后中性粒细胞 (PMN)表面CD18、肺组织ICAM 1表达的影响。方法 72只大鼠随机化方法分为假手术组、缺血再灌注组、PTX组。采用肺在体温缺血再灌注损伤模型 ,于缺血 4 5min、再灌注 1、2、4h测定肺组织含水量、支气管肺泡灌洗液 (BALF)白蛋白含量、肺组织及BALF髓过氧化物酶 (MPO)活性、流式细胞仪测定CD18 FITC标记的PMN阳性细胞百分率 ,肺组织ICAM 1免疫组化染色 ,并进行图像分析。结果 缺血再灌注组再灌注后各时相点肺含水量、BALF白蛋白含量、左肺组织及BALFMPO活性及CD18、ICAM 1表达均有非常显著的升高 (P <0 .0 1) ,PTX则可以明显抑制这种变化。结论 PTX可能通过降低肺缺血再灌注引起的CD11 CD18及ICAM 1的表达 ,而减轻PMN在肺内的聚集、活化 ,从而防治肺缺血再灌注损伤。
Objective To investigate the effects of pentoxifylline on the expression of CD18 on lung neutrophils (PMN) and the expression of ICAM 1 in lung tissue after lung ischemia / reperfusion injury in rats. Methods 72 rats were randomly divided into sham operation group, ischemia reperfusion group and PTX group. The model of ischemia-reperfusion injury in the lungs was used to measure the lung water content, albumin content in bronchoalveolar lavage fluid (BALF), lung tissue and myeloperoxide in BALF (MPO) activity, the percentage of CD18 FITC labeled PMN positive cells and the expression of ICAM 1 in lung tissue were determined by flow cytometry, and the images were analyzed. Results After reperfusion, the lung water content, BALF albumin content, left lung and BALFMPO activity and the expressions of CD18 and ICAM 1 in ischemia / reperfusion group were significantly increased (P <0.01) PTX can significantly inhibit this change. Conclusions PTX can prevent lung ischemia-reperfusion injury by reducing the expression of CD11 CD18 and ICAM-1 caused by lung ischemia-reperfusion and decreasing the aggregation and activation of PMN in the lung.