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目的:分析激肽释放酶10(kallikrein 10,KLK10)和血管内皮生长因子(vascular endothelial growth factor,VEGF)在卵巢癌组织中的表达,探讨两者在卵巢癌临床诊断、治疗及预后中的意义。方法:收集2004年1月至2009年1月在南通大学附属医院妇科收治的45例卵巢癌、10例良性和12例交界性卵巢肿瘤组织石蜡切片标本,应用免疫组化法检测标本中KLK10蛋白和VEGF的表达,并分析两者表达的相关性及与卵巢癌各临床病理指标和预后的关系。结果:KLK10[86.7%(39/45)vs 10.0%(1/10)、58.3%(7/12),P<0.05]和VEGF[(82.2%(37/45)vs 20.0%(2/10)、41.4%(5/12),P<0.05]在卵巢癌组织中的阳性表达率均明显高于卵巢良性、交界性肿瘤组织。KLK10和VEGF表达阳性率分别与分期、肿瘤分化、淋巴结转移、5年生存率有关(P<0.05),与患者年龄、病理分型、血清CA125水平、腹水及残余肿瘤直径无关(P>0.05);KLK10和VEGF蛋白在卵巢癌中的表达呈正相关性(r=0.5279,P=0.043)。结论:KLK10和VEGF蛋白在卵巢癌中均为高表达,两者表达呈正相关,两者均似可作为卵巢癌诊断、治疗及预后的标志物。
OBJECTIVE: To analyze the expression of kallikrein 10 (KLK10) and vascular endothelial growth factor (VEGF) in ovarian cancer, and to explore their clinical significance in the diagnosis, treatment and prognosis of ovarian cancer . Methods: A total of 45 cases of ovarian cancer, 10 cases of benign and 12 cases of borderline ovarian tumor were collected from gynecology department of Nantong University Affiliated Hospital from January 2004 to January 2009. Immunohistochemistry was used to detect the expression of KLK10 And VEGF expression, and analyze the correlation between the expression and the relationship with the clinicopathological parameters and prognosis of ovarian cancer. Results: The percentage of patients with KLK10 [86.7% (39/45) vs 10.0% (1/10), 58.3% (7/12), P <0.05] and VEGF [82.2% (37/45) vs 20.0% ), 41.4% (5/12), P <0.05] in ovarian cancer tissues were significantly higher than those in benign and borderline ovarian cancers.The positive rates of KLK10 and VEGF were correlated with stage, tumor differentiation, lymph node metastasis (P <0.05), and had no correlation with age, pathological type, serum CA125 level, ascites and residual tumor diameter (P> 0.05). The expression of KLK10 and VEGF in ovarian cancer was positively correlated r = 0.5279, P = 0.043) .Conclusion: Both KLK10 and VEGF proteins are highly expressed in ovarian cancer, and the expression of KLK10 and VEGF is positively correlated with each other, both of which may serve as markers for the diagnosis, treatment and prognosis of ovarian cancer.