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为了更好地利用生物技术手段提高观赏凤梨的开花质量,本研究在分析蜻蜓凤梨转录组数据的基础上,根据同源性比对发现了一个类TERMINAL FLOWER 1(TFL1)基因的c DNA片段,结合c DNA末端快速扩增(rapid amplification of c DNA ends,RACE)技术获得了其937 bp的c DNA全长,并将其命名为Af TFL1。序列分析表明,该基因的开放阅读框为522 bp,编码173个氨基酸。蛋白质性质分析表明,其分子量为19.329 9 k D,等电点为8.39,为一类不稳定的碱性蛋白。结构域分析表明,此蛋白含有1个典型的PEBP结构域。系统进化树分析表明,Af TFL1与番红花的TFL1亲缘关系最近。实时荧光定量PCR结果表明,Af TFL1在蜻蜓凤梨各个部位的表达量不同,在营养器官中的表达量随株龄增长而降低,在生殖器官中表达量非常低甚至检测不到。研究结果可为进一步研究Af TFL1的功能以及深入探讨蜻蜓凤梨开花机理提供了一定的理论依据。
In order to improve the flowering quality of ornamental pineapple by means of biotechnology, this study found a c DNA fragment of TERMINAL FLOWER 1 (TFL1) gene based on the homology comparison based on the data of the dragonfly pineapple transcriptome. The full-length cDNA of 937 bp was obtained by rapid amplification of c DNA ends (RACE) technique and named Af TFL1. Sequence analysis showed that the open reading frame of the gene was 522 bp, encoding 173 amino acids. The analysis of protein properties showed that the molecular weight was 19.329 9 kD and the isoelectric point was 8.39, which was a kind of unstable basic protein. Domain analysis showed that this protein contains a typical PEBP domain. Phylogenetic tree analysis showed that Af TFL1 was closest to TFL1 in saffron. Real-time PCR results showed that Af TFL1 expressed differently in all parts of the dragonfly pineapple and its expression in vegetative organs decreased with the growth of the plant, and its expression level in reproductive organs was very low or undetectable. The results may provide some theoretical basis for further study of the function of Af TFL1 and in-depth discussion of the flowering mechanism of the dragonfly pineapple.