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目的:研究盐酸氨基胍(AG)对兔牙扭转移动过程中牙周组织内破骨细胞的影响,并分析其机制。方法:选取36只雄性新西兰大白兔,随机分为2组,建立兔正畸牙扭转移动模型。分别于扭转牙颊侧局部骨膜下注射40mg/mL盐酸氨基胍(AG组)和生理盐水(NaCl组),隔天1次。于加力后第3、7、14、21、28和42天处死动物,取材制作切片,行TRAP酶组织化学和诱导型一氧化氮合酶(iNOS)免疫组织化学染色,观察破骨细胞募集以及iNOS的表达情况。采用SPSS16.0软件包对数据进行统计学分析。结果:2组破骨细胞募集数量变化趋势具有相似规律。与NaCl组相比,AG组自第14天起破骨细胞募集数量显著降低(P<0.05)。自加力第21天起,AG组牙周组织压力侧iNOS表达的阳性面积百分比显著小于NaCl组(P<0.05)。AG组破骨细胞计数与iNOS阳性表达面积百分比呈正相关(r=0.875,P<0.05)。结论:AG对iNOS的表达有抑制作用,可能是通过减少NO生成而间接抑制破骨细胞的募集。
Objective: To study the effect of aminoguanidine hydrochloride (AG) on periodontal tissue osteoclasts in the process of torsion movement and to analyze the mechanism. Methods: Thirty-six male New Zealand white rabbits were randomly divided into 2 groups to establish a model of orthodontic tooth torsion. The rats were injected subcutaneously with 40mg / mL aminoguanidine hydrochloride (AG) and normal saline (NaCl group) subcutaneously on the buccal side of the tooth respectively, once every other day. The animals were sacrificed on days 3, 7, 14, 21, 28 and 42 after the animals were harvested for slicing. TRAP enzymatic histochemistry and inducible nitric oxide synthase (iNOS) immunohistochemical staining were performed to observe the osteoclast recruitment As well as iNOS expression. SPSS16.0 software package for statistical analysis of the data. Results: The trend of the number of recruited osteoclasts in two groups had similar rules. Compared with NaCl group, the number of osteoclasts raised from day 14 in AG group was significantly decreased (P <0.05). From the 21th day after loading, the positive area percentage of iNOS on the pressure side of periodontal tissue in AG group was significantly lower than that in NaCl group (P <0.05). AG group osteoclast count and iNOS positive expression area percentage was positively correlated (r = 0.875, P <0.05). Conclusion: AG inhibits the expression of iNOS, which may indirectly inhibit the recruitment of osteoclasts by reducing NO production.