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目的:观察蒿甲醚(Art)对日本血吸虫磷酸化酶(PP)、乳酸脱氢酶(LDH)、6-磷酸葡萄糖脱氢酶(G-6-PDH)和三磷酸腺苷酶(ATPase)的影响。方法:感染32-38天的小鼠于灌服Art 100-300mg·kg~(-1)后24-72h剖杀,收集雌()、雄虫(),按NADH和NADPH的形成和无机磷的释放量测定虫的上述4种酶。结果:感染小鼠用Art 300mg·kg~(-1)治疗后24-48h,、虫的总PP和PPa(激活型)活力明显增加,而PPb则无或仅有轻度增加。上述小鼠用Art 100-300mg·kg~(-1)治疗后24-72h,LDH和G-6-PDH的抑制率分别为9％-59％()和41％-75％()及22％-42％()和74%-80%()。用300mg·kg~(-1)治疗后24h,仅虫的Mg~2-ATPase明显受抑制,48h后,Ca~(2+)-ATPase、Mg~(2+)-ATPase和Na~+-K~+-ATPase分别抑制17％()和19％(),32％()和48％(),及29％()和44％()。结论:Art引起血吸虫PPa活力的增加,使虫的糖原分解,并抑制LDH使虫糖酵解的终产物乳酸明显减少。此外,对血吸虫虫的G-6-PDH有明显的抑制作用。
OBJECTIVE: To observe the effects of Artemether on Schistosoma japonicum phosphorylase (PP), lactate dehydrogenase (LDH), glucose 6-phosphate dehydrogenase (G-6-PDH) and ATPase. Methods: Mice infected 32-38 days after Art 100-300mg · kg -1 were killed 24-72h and female (), male () were collected. According to the formation of NADH and NADPH and inorganic phosphorus The amount of the release of the determination of insects the above four kinds of enzymes. RESULTS: Twenty-four to forty-eight hours after treatment with Art 300 mg · kg -1, the total PP and PPa (activated) activity of the infected mice increased significantly, while PPb showed no or only slight increase. The inhibitory rates of LDH and G-6-PDH were 9% -59% () and 41% -75% () and 22% respectively after 24-72h treatment with Art 100-300mg · kg -1 % -42% () and 74% -80% (). After treatment with 300 mg · kg -1 for 24 h, only the Mg 2 - ATPase in worms was significantly inhibited. After 48 h, the contents of Ca 2 + - ATPase, Mg 2 + - ATPase and Na + K + -ATPase inhibited 17% and 19%, 32% and 48%, respectively, and 29% and 44%, respectively. Conclusion: Art caused the increase of PPa activity of Schistosoma japonicum, which decomposed the glycogen of insect and inhibited the lactic acid of the terminal product of insect glycolytic. In addition, Schistosoma japonicum G-6-PDH significantly inhibited.