甘蓝黑癌病菌能浸染多种十字花科蔬菜,包括叶用甘蓝、球茎甘蓝、抱子甘蓝、大白菜、油菜、芥菜、萝卜等。种子带菌是黑癌病广泛传播的重要原因。华盛顿州是美国生产十字花科蔬菜种子的重要基地,占美国生产总量的80%,其甘蓝种子销售量占世界30%左右。近年已发现种子田中有黑腐病危害,威胁种子生产和出口贸易,为此加强种子检验技术和种子消毒技术的研究,值得重视。种子检验技术将甘蓝黑腐病病叶研磨后按一定量混入种子中,放在冰箱内备用。用热水浸泡沫(50℃、30分钟)或用其他药物处理种子。处理后的种子自然干燥1～2天,用SX琼脂平板(Schaad,N.W等人1974年)和肉汁淀粉放线菌酮琼脂平板(Schaad,N.W等人1975年)直接培养种子,经30℃、10天,即可用肉眼直接观察种子周围的菌落色泽和 Cabbage black bacteria can dip a variety of cruciferous vegetables, including leaf cabbage, kohlrabi, Brussels sprouts, cabbage, rape, mustard, radish and so on. Seed carrier is an important cause of widespread spread of black cancer. Washington State is an important base for producing cruciferous vegetable seeds in the United States, accounting for 80% of the total U.S. production. Its sales of cabbage seeds account for about 30% of the world’s total. In recent years, it has been found that there is a threat of black rot in the seed field, threatening the production and export of seeds. Therefore, it is worth to pay attention to strengthening the research on seed testing technology and seed disinfection technology. Seed testing technology will be cabbage black rot disease leaves after grinding by a certain amount of mixed seeds, placed in the refrigerator spare. Soak the foam with hot water (50 ° C for 30 minutes) or treat the seeds with other drugs. The treated seeds were naturally dried for 1 to 2 days and seeds were directly cultivated with SX agar plates (Schaad, NW et al., 1974) and gramicidin, cycloheximide agar plates (Schaad, NW et al., 1975) 10 days, you can directly observe with the naked eye colony color and seed around