利用5%、10%、15%、20%、25%、30%浓度的二甲基亚砜和甘油肝浸汤培养液对临床分离的阴道毛滴虫进行4℃保存观察。二甲基亚砜和甘油两实验组最适保种浓度分别为15%和10%,50%虫体存活率天数分别为29和27 d,最长保存天数分别为31和29 d;而空白对照组仅为5和7 d。结果表明,15%二甲基亚砜或10%甘油肝浸汤培养液4℃冷藏保存阴道毛滴虫可显著延长其保存期。 Clinically isolated Trichomonas vaginalis was maintained at 4 ° C using 5%, 10%, 15%, 20%, 25%, and 30% DMSO and glycerol liver immersion broth. The optimum preservative concentrations of dimethyl sulfoxide and glycerol were 15% and 10% respectively, and the survival rates of 50% worms were 29 and 27 days respectively, with the longest days of preservation being 31 and 29 days respectively. The control group was only 5 and 7 days. The results showed that cryopreservation of Trichomonas vaginalis with 15% dimethyl sulfoxide or 10% glycerol liver immersion broth at 4 ℃ could prolong the storage period.