Beiling water vesicie method is more simple than traditional mixing methods and water vapour method to purify tortoise plastron. The chromatogtams of amino acids obtsined were similar. The water extract or 50% alcohol exact by boiling water vesle method is more than those by mixing method and is similar to those by water vapour method. The water vesicie method is more simple than traditional mixing methods and water vapour method to purify tortoise plastron. The chromatogtams of amino acids obtsined were similar. The water extract or 50% alcohol exact by boiling water vesle method is more than those by mixing method and Is similar to those by water vapour method.