论文部分内容阅读
目的探讨帕金森病多巴胺受体在疾病状态下超微结构上的分布变化。方法用免疫组织化学和包埋前免疫胶体金电镜方法,研究6-羟多巴胺(6-OHDA)单侧损毁大鼠的多巴胺能通路帕金森病模型,在4周和16周时纹状体D1多巴胺受体(D1R)和D2多巴胺受体(D2R)的变化。结果光镜观察显示,4周时损伤侧纹状体D1R减少,在电镜下可见该减少在棘突而不是树突或胞体;16周时,光镜下虽然损伤侧D1R恢复到损伤对侧水平,但在电镜下却可见损伤侧棘突和树突D1R数量减少,而胞体的D1R数量却明显增多。4周和16周时损伤侧D2R在光镜下均增多,在电镜下D2R胶体金颗粒增多主要发生在棘突和树突而不是胞体。另外,损伤后4周和16周,可见这两种受体的金颗粒更多地位于突触外。结论这些变化可能在帕金森病症状发生发展及左旋多巴治疗副作用的产生中起重要作用。
Objective To investigate the ultrastructural distribution of dopamine receptors in Parkinson’s disease. Methods Immunohistochemistry and immunocytochemistry were used to study the dopaminergic pathological model of Parkinson’s disease induced by 6-hydroxydopamine (6-OHDA) unilateral lesion in rats. At week 4 and week 16, striatum D1 Changes in dopamine receptor (D1R) and D2 dopamine receptor (D2R). Results Light microscopy showed that D1R decreased in injured striatum at 4 weeks. This decrease was observed under electron microscopy in the spinous process but not in dendrites or cell bodies. At week 16, although D1R was restored to the lesion contralateral level However, under the electron microscope, the number of denervated spinous processes and dendrites D1R decreased while the number of D1Rs in the somatic cells increased significantly. At 4 and 16 weeks, the D2R on the injured side increased under light microscope. The increase of D2R colloidal gold particles mainly occurred in the spinous processes and dendrites rather than the cytoplasm under electron microscopy. In addition, 4 weeks and 16 weeks after injury, it can be seen that the gold particles of these two receptors are located more outside the synapse. Conclusion These changes may play an important role in the development of Parkinson’s disease symptoms and the side effects of levodopa therapy.