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目的 探讨胃癌病人肿瘤坏死因子 (TNF) α 30 8基因多态性与癌性恶液质的关系。方法 该研究包括 78例营养不良的胃癌病人 ,按照近 6个月体重的减轻率分成 3组 :A组 ,<5 % ;B组 ,5 %～ 10 % ;C组 ,>10 %。用多聚酶链反应 (PCR)结合NcoI限制性内切酶检测胃癌病人及 89位正常志愿者的TNF 30 8位点。结果 TNF2出现的频率在胃癌组和对照组的差异无显著性 (P =0 781)。胃癌病人TNF2组和TNF1组恶液质的发病率差异无显著性 (P =0 111) ;血浆TNF α浓度在A、B、C三组差异有显著性 (P <0 0 1) ;TNF2出现的频率在A、B两组差异无显著性 (P >0 0 5 ) ,C组与A组比较 ,C组与B组比较差异皆有显著性 (P =0 0 11和P =0 0 14 )。结论 TNF α 30 8基因多态性与重度恶液质的致病过程有相关性。
Objective To investigate the relationship between tumor necrosis factor (TNF) α 30 8 gene polymorphism and cancerous and malignant fluid in patients with gastric cancer. Methods The study included 78 patients with malnourished gastric cancer who were divided into 3 groups according to the weight loss rate of the past 6 months: group A, <5%; group B, 5% -10%; group C,> 10%. The TNF30 8 locus in gastric cancer patients and 89 normal volunteers was detected by polymerase chain reaction (PCR) combined with NcoI restriction enzyme. Results The frequency of TNF2 expression was not significantly different between gastric cancer group and control group (P = 0 781). There were no significant differences in the incidence of catecholamines between the TNF2 group and the TNF1 group (P = 0 111). There was a significant difference in the levels of TNFα among the three groups (P <0.01) There was no significant difference in the frequency of A and B between the two groups (P> 0.05). There was significant difference between group C and group A (P = 0 0 11 and P = 0 0 14 ). Conclusion The TNF α 30 8 gene polymorphism is associated with the pathogenesis of severe cachexia.