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对所用的受体载体、克隆位点、感受态细胞的制备、转化方法、以及质粒的提取方法进行了筛选,建立了一套适于外源基因克隆、转化的体系,为下一步将转基因产品中常见外源基因转化到同一受体菌中做好准备。
The receptor vectors, cloning sites, preparation of competent cells, transformation methods and plasmid extraction methods were screened and a set of suitable cloning and transformation system of exogenous genes was established. In the next step, the transgenic products In the common foreign genes into the same recipient bacteria ready.