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目的探讨3.0T MRI化学交换饱和位移成像技术(chemical exchange saturation transfer,CEST)对肌酸代谢成像的可行性及相关影响因素。方法准备不同浓度肌酸及其代谢产物的体外模型和不同pH值的肌酸及其对照组体外模型。采用3.0T MRI对这两组模型进行CEST成像,通过CEST效应及Z谱进行量化分析。结果 3.0T MRI可检测到不同浓度肌酸之间信号(±1.8ppm)差异明显(F=99.08,P<0.001),且信号强度随着浓度增加而增强(r=0.963,P<0.001)。另外,不同pH值环境CEST效应差异明显(F=5.539,P=0.002),信号随pH值下降而逐渐减低(r=0.41,P=0.035)。结论 3.0T MRI进行CEST成像评价肌酸代谢是可行的,且其CEST效应受肌酸浓度及代谢环境pH值等因素影响。
Objective To investigate the feasibility of 3.0T MRI chemical exchange saturation transfer imaging (CEST) on creatine metabolism imaging and related influential factors. Methods In vitro models of creatine and its metabolites with different concentrations and creatine of different pH values and their control groups were prepared in vitro. CET imaging was performed on these two models using 3.0T MRI and quantified by CEST effect and Z-spectrum. Results The signal intensity of creatine at different concentrations (± 1.8ppm) was significantly different at 3.0T MRI (F = 99.08, P <0.001), and the signal intensity increased with the increase of concentration (r = 0.963, P <0.001). In addition, the CEST effect was significantly different at different pH values (F = 5.539, P = 0.002). The signal decreased with the pH value decreasing (r = 0.41, P = 0.035). Conclusion CEST imaging of creatine metabolism by 3.0T MRI is feasible and its CEST effect is affected by creatinine concentration and metabolic pH value.