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烟草环斑病毒是我国检疫性有害生物,为防止该病毒传入国内,需建立快速的诊断和检测方法。将烟草环斑病毒的多克隆抗体吸附到磁性纳米颗粒上,研制出磁免疫层析检测试纸条。该试纸条可特异性检测烟草环斑病毒,检测部分提纯病毒的灵敏度为1μg/mL,检测烟草病汁液可稀释1000倍(重量/体积)。该试纸条和线虫传多面体病毒属的番茄环斑病毒、番茄黑环病毒、南芥菜花叶病毒和葡萄扇叶病毒未出现交叉反应,可用于烟草环斑病毒的现场检测。
Tobacco ring spot virus is a quarantine pest in our country. In order to prevent the virus from being introduced into China, a rapid diagnosis and detection method should be established. The polyclonal antibody of tobacco ring spot virus was adsorbed on the magnetic nanoparticles, and a magnetic immunochromatographic test strip was developed. The strip can be used for the detection of tobacco ring spot virus. The detection sensitivity of the partially purified virus is 1μg / mL. The test tobacco juice can be diluted 1000 times (weight / volume). The test strip and the nematode polyhedrosis Tomato ring spot virus, tomato black ring virus, Arabidopsis mosaic virus and grapevine leaf virus cross-reaction does not occur, can be used for tobacco ring spot virus on-site testing.