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目的：用卵巢癌抗独特型单链抗体６Ｂ１１ｓｃＦｖ代替肿瘤抗原免疫动物，观察是否诱导动物产生特异性体液免疫反应。方法：将６Ｂ１１ｓｃＦｖ交联钥孔虫戚虫血蓝素，辅以弗氏佐剂反复免疫ＢＡＬＢ／ｃ小鼠，制备抗血清。采用间接法ＥＬＩＳＡ，竞争抑制ＥＬＩＳＡ及免疫流式细胞法分析抗血清特性。结果：经ＥＬＩＳＡ检测显示，由６Ｂ１１ｓｃＦｖ刺激产生的抗抗独特型单链抗体（Ａｂ３）能与６Ｂ１１ｓｃＦｖ和卵巢癌组织抗原ＯＣ１６６９特异性结合。竞争抑制ＥＬＩＳＡ表明，Ａｂ３能有效抑制卵巢癌单抗ＣＯＣ１６６９（Ａｂ１）与ＯＣ１６６９的特异性结合。免疫流式分析结果可见，Ａｂ３能与表达卵巢癌抗原的人卵巢癌细胞系ＯＶ１细胞表面结合而不能与无卵巢癌抗原表达的人宫颈癌细胞系ＨｅＬａ细胞表面结合。从以上结果可以证明Ａｂ３与Ａｂ１的抗原结合特性相同。结论：６Ｂ１１ｓｃＦｖ能代替卵巢癌抗原诱导动物产生特异性体液免疫反应，具有模拟抗原的作用，为６Ｂ１１ｓｃＦｖ作为卵巢癌抗独特型单链抗体疫苗的实际应用提供依据
OBJECTIVE: To use ovine cancer anti-idiotypic single chain antibody 6B11scFv instead of tumor antigen immunized animals to observe whether inducing specific humoral immune response in animals. Methods: BALB / c mice were immunized repeatedly with 6B11scFv cross-linked keyhole limpet hemocyanin with Freund’s adjuvant to prepare antiserum. Indirect ELISA, competitive inhibition ELISA and immunofluorescence were used to analyze the antiserum characteristics. Results: The results of ELISA showed that anti- idiotypic single chain antibody (Ab3) stimulated by 6B11scFv could specifically bind to 6B11scFv and ovarian cancer antigen OC1669. Competitive inhibition ELISA showed that Ab3 can effectively inhibit the specific binding of ovarian cancer monoclonal antibody COC166-9 (Ab1) and OC166-9. Immunofluorescence analysis showed that Ab3 could bind to the surface of human ovarian cancer cell line OV1 expressing ovarian cancer antigen but not to the human cervical cancer cell line HeLa cell without ovarian cancer antigen expression. From the above results, it can be proved that the antigen-binding properties of Ab3 and Ab1 are the same. CONCLUSION: 6B11scFv can substitute for ovarian cancer antigen to induce specific humoral immune response in animals and has the function of mimicking antigens. This provides a basis for the practical application of 6B11scFv as an anti-idiotypic single chain antibody against ovarian cancer