背景:海藻具有广阔的药理活性前景,加强其研究对有目的进行应用开发有重要的指导意义。目的:观察海洋单细胞海藻在体外对人脑胶质瘤干细胞生物学活性的影响。方法:以酶消化法培养人脑胶质瘤干细胞,流式细胞分选出CD133阳性细胞,细胞传代获得第3代细胞。流式细胞仪检测海洋单细胞海藻作用前后细胞CD133表达变化,免疫组织化学检测贴壁细胞巢蛋白及胶质纤维酸性蛋白的表达。实验组分别加入不同质量浓度的海洋单细胞海藻,阴性对照加不含药的PBS,将稀释成4,6,8,10g/L的海洋单细胞海藻加入细胞培养液中并作用24,48,72h,流式细胞仪检测胶质瘤干细胞生长周期,应用酶标仪检测细胞生长抑制情况。结果与结论:随着浓度和时间的增加,与对照组相比倒置显微镜下可见实验组胶质瘤干细胞不易聚团成球,出现贴壁分化,并逐渐明显;加药后胶质瘤干细胞CD133表达量明显减少;出现的贴壁细胞免疫组织化学染色巢蛋白及胶质纤维酸性蛋白表达阳性;流式细胞仪检测显示,停滞在S、G2/M期细胞数增加,而G0/G1期细胞数减少;随着浓度和时间的增加,胶质瘤干细胞增殖明显抑制,与对照组相比差异有显著性意义(P<0.05～0.01)。提示海洋单细胞海藻能够抑制人脑胶质瘤干细胞的增殖,并促进其分化,且作用具有浓度和时间依赖性。 Background: Seaweed has a broad prospect of pharmacological activity. It is important to strengthen the research on the purposeful application and development. Objective: To observe the effects of marine single-cell seaweed on the biological activity of human glioma stem cells in vitro. Methods: Human glioma stem cells were cultured by enzymatic digestion, and CD133 positive cells were sorted by flow cytometry. The third passage cells were obtained by passage. Flow cytometry was used to detect the expression of CD133 in the cells before and after the action of marine single-cell algae. The expression of adherent cells nestin and glial fibrillary acidic protein was detected by immunohistochemistry. Experimental groups were added different concentrations of marine single-cell seaweed, negative control plus drug-free PBS, seaweed diluted to 4,6,8,10 g / L of marine cells into the cell culture medium and the role of 24,48, 72h, flow cytometry of glioma stem cell growth cycle, the use of microplate reader to detect cell growth inhibition. RESULTS AND CONCLUSION: As concentration and time increased, compared with the control group, the experimental group showed that the glioma stem cells were not easy to agglomerate and appeared adherent differentiation, and gradually became apparent. Compared with the control group, glioma stem cells CD133 The expression of Nestin and glial fibrillary acidic protein in adherent cells was significantly lower than that in control group. Flow cytometry showed that the number of cells arrested in S, G2 / M phase increased, while in G0 / G1 phase cells With the increase of concentration and time, the proliferation of glioma stem cells was significantly inhibited compared with the control group (P <0.05 ~ 0.01). It is suggested that marine single-cell algae can inhibit the proliferation and promote the differentiation of human glioma stem cells in a concentration- and time-dependent manner.