论文部分内容阅读
目的观察邻苯二甲酸二(2-乙基己基)酯(DEHP)对大鼠的生殖发育毒性作用。方法采用围生期毒性试验的研究方法,将清洁级SD大鼠随机分为对照组(玉米油)和4个DEHP染毒组(125、250、500、1000mg/kg),采用宫内及哺乳期经口灌胃的方式染毒。记录比较孕鼠孕0、20天(GD0、GD20)、仔鼠出生后21天(PND21)母鼠体重及体重增长量,产后第1天(PND1)记录产仔总数及仔鼠平均出生体重;产后第4天(PND4)辨别雌雄仔鼠,分别测量雌雄仔鼠平均肛殖距,于仔鼠断乳(PND21)后,计数着床数,取母鼠各脏器称重并计算脏器系数。结果母鼠染毒期间,体重及其体重增长量不同程度降低(P<0.05),各脏器系数均不同程度增大(P<0.01),各染毒组受孕率差异有统计学意义(χ2=16.816,P<0.01);与对照组比较,1000mg/kg组仔鼠平均活胎数、平均出生体重、子宫着床点数降低(P<0.05),肛殖距不同程度缩短(P<0.01)。结论DEHP可降低雌鼠受孕率及母鼠产仔总数,可通过胎盘屏障对仔鼠的生长发育产生毒性作用,导致低出生体重,对仔鼠的生殖发育有毒性作用。
Objective To observe the toxic effects of DEHP on reproductive development in rats. Methods The method of perinatal toxicity test was used to divide clean SD rats into control group (corn oil) and four DEHP groups (125, 250, 500 and 1000 mg / kg) Period by oral gavage poisoning. The body weight and body weight gain of pregnant rats at day 0 and day 20 (GD0 and GD20), the pups’ birth weight and body weight at 21 days after birth (PND21) were recorded. The total number of littermates and the average birth weight of pups were recorded on the first day postpartum (PND1) Male and female offspring were identified on the fourth day postpartum (PND4), and the average anal intervertebral distance was measured in male and female offspring respectively. After PND21, the number of implants was counted. The organs were weighed and the organ coefficient . Results The body weight and body weight gain of female rats decreased to different extents (P <0.05), and the coefficients of various organs increased to different extents (P <0.01), and there was significant difference in pregnancy rate = 16.816, P <0.01). Compared with the control group, the average number of live births, average birth weight, the number of implantation sites in the uterus in 1000mg / kg group were significantly decreased (P <0.05) . Conclusion DEHP can reduce the pregnancy rate of female rats and the total number of litters, which may have toxic effects on the growth and development of offspring through the placental barrier, resulting in low birth weight and toxic effects on reproductive development of pups.