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目的:本研究评价了青阳参总苷(QYS)在慢性不可预知轻度应激(CUMS)大鼠模型上的抗抑郁活性,并通过研究其对大鼠脑内单胺神经递质浓度的影响探讨QYS的作用机制。方法:将Wistar大鼠随机分为6组,分别为正常组、模型组、氟西汀组和QYS低、中、高剂量组(0.625,2.5,10 mg·kg-1),每组9只。随机给予21 d不可预知刺激建立抑郁症模型,建模同时ig给予相应药物。造模结束后,采用旷场实验观察QYS对大鼠自主活动的影响,采用悬尾实验、强迫游泳实验观察QYS的抗抑郁作用,采用高效液相-电化学检测法测定大鼠脑内单胺递质去甲肾上腺素(NE),5-羟色胺(5-HT)及多巴胺(DA)的浓度。结果:与正常组比较,模型组大鼠悬尾时间及大鼠强迫游泳的不动时间明显延长,自主活动时间明显减少;大鼠脑内额叶皮层中NE,5-HT及DA的浓度明显降低,均具有明显的统计学差异(P<0.05,P<0.01);与模型组比较,QYS(0.625,2.5,10mg·kg-1)能够显著缩短模型大鼠悬尾实验中的不动时间,QYS高剂量组显著缩短模型组大鼠强迫游泳实验中的不动时间,QYS在旷场实验中对模型组大鼠的自主活动无明显影响,QYS高剂量组显著升高模型组大鼠脑内额叶皮层中NE,5-HT及DA的浓度。结论:QYS高剂量对模型抑郁大鼠具有良好的抗抑郁活性,其抗抑郁作用机制可能是QYS提高了模型大鼠脑内额叶皮层NE及5-HT水平。
OBJECTIVE: This study evaluated the antidepressant activity of QYS in a rat model of chronic unpredictable mild stress (CUMS), and by studying its effect on monoamine neurotransmitter concentration Impact on the mechanism of QYS. Methods: Wistar rats were randomly divided into 6 groups: normal group, model group, fluoxetine group and QYS low, medium and high dose groups (0.625, 2.5 and 10 mg · kg-1) . A 21-day unpredictable stimulus was given randomly to establish a model of depression, while modeling was given to the appropriate drug. After modeling, the open field test was used to observe the effect of QYS on autonomic activity in rats. Anti-depression effects of QYS were observed by force-swimming test and tail-suspension test. Monoamine Neurotransmitters (NE), serotonin (5-HT) and dopamine (DA) concentrations were measured. Results: Compared with the normal group, the immobility time of rats in forced swimming and prolonged spontaneous activity in model group were significantly prolonged, and the concentrations of NE, 5-HT and DA in the frontal cortex of rats were significantly increased (P <0.05, P <0.01). Compared with the model group, QYS (0.625,2.5 and 10 mg · kg-1) significantly shortened the immobility time in tail-suspension test in model rats QYS high dose group significantly shortened immobility time in forced swimming test in model group, QYS had no significant effect on autonomic activity of model group rats in open-field test, QYS high dose group significantly increased rat brain Concentrations of NE, 5-HT and DA in the prefrontal cortex. Conclusion: QYS high dose has good antidepressant activity on depressive rats, and its antidepressant mechanism may be that QYS increases the level of NE and 5-HT in frontal cortex of model rats.