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采用敏感的酶联免疫吸附试验，对４例异种表面角膜镜片术和３例异种穿透性角膜移植术（鹅给猴）术后血清特异性抗体水平的变化进行了动态的观察。发现表面镜片术组于木后第１周出现特异性抗体，术后第３周达高峰，第４周明显下降，穿透性角膜移植术组也于木后第１用出现特异性抗体，第６周达高峰，术后第８周开始下降。临床观察发现４例表面角膜镜片术的木眼于术后１８～２５天出现角膜新生血管，并侵入植片的周边部，拆线后３例术８眼角膜新生血管消退，植片透明，１例因层间上皮植入角膜新生血管未消退，植片轻度水肿混浊。３例穿透角膜移植术的术眼于术后１４～１７天出现角膜新生血管，术后６周植片完全混浊。以上结果表明不仅新鲜角膜组织能诱发受主的体液免疫反应，经过冷冻切削的角膜组织也能诱发机体的体液免疫反应。
Sensitive enzyme-linked immunosorbent assay (ELISA) was used to observe the changes of serum specific antibody levels in 4 cases of heterosexual keratomileusis and 3 cases of hereditary penetrating keratoplasty. Found that the surface of the lens surgery group in the first week after the emergence of specific antibodies, after 3 weeks reached its peak, decreased significantly after 4 weeks, penetrating keratoplasty was also used in the first wood specific antibodies, the first 6 weeks reached its peak, began to decline after 8 weeks. Clinical observation showed that corneal neovascularization occurred in 4 cases of corneal surface ophthalmoscopy 18 to 25 days postoperatively and invaded the periphery of the graft. Three cases of corneal neovascularization were cured in 8 cases Cases of corneal neovascularization due to interstitial epithelial implantation, subtle opacity. Corneal neovascularization occurred in 3 cases of penetrating keratoplasty at 14 to 17 days after operation, and complete opacification at 6 weeks after operation. The above results show that not only fresh corneal tissue can induce the humoral immune response of the recipient, after frozen corneal tissue can also induce the body’s humoral immune response.