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临床角膜标本150例经锥兰,茜素红活体染色后测定角膜周边与中央内皮细胞的相对密度以及进行内皮细胞核形分析。结果表明,25例19周至34周胎儿的角膜中央和周边内皮细胞密度无显著性差别(P>0.05),而19例新生儿至53岁的角膜内皮细胞密度在角膜中央与边缘的测定数值有显著性差异(P<0.01)。胎儿角膜内皮细胞的细胞核以椭园形为主,角膜周边与中央区的细胞核形状比较相似,都处于不稳定的核形结构。出生后至成年的角膜内皮细胞核形趋于稳定,以园形为主,但在角膜缘总仍散在不稳定的椭园形细胞核,它揭示成年体角膜缘部的内皮细胞仍有部份保留着分化功能。
The clinical corneal specimens of 150 cases were treated with pycnogenol and alizarin red, and the relative density of corneal peripheral and central endothelial cells was determined. The endothelial cell nuclear shape was also analyzed. The results showed that there was no significant difference in the density of corneal central and peripheral endothelial cells in 25 fetuses from 19 weeks to 34 weeks (P> 0.05), while the values of corneal endothelium in 19 neonates to 53 years old were Significant difference (P <0.01). Fetal corneal endothelial cell nucleus to oval-shaped, corneal peripheral and central region of the nuclear shape is relatively similar, are in unstable nuclear structure. The nucleus of corneal endothelial cells from birth to adulthood tended to be stable, mainly in the park. However, the corneal limbus was still scattered in the unstable elliptical nucleus. It revealed that some of the endothelial cells in the corneal limbus still remained in the adult body Differentiation function.