论文部分内容阅读
目的研究在粗化纯钛表面采用仿生钙磷涂层法组装丹参的可行性。方法钛片表面粗化,采用仿生钙磷涂层技术,在纯钛表面建立不同浓度含丹参涂层,通过场发射扫描电子显微镜(FSEM)、傅立叶红外光谱(FTIR)、X射线衍射仪(XRD)和元素分析仪观察涂层晶体形貌、化学结构及组成,通过细胞培养验证其生物活性。结果 FSEM证实低浓度的丹参对钙磷涂层晶体的生长影响不大,高浓度的丹参对钙磷涂层晶体的生长影响较大;XRD和FTIR表明丹参并没有破坏涂层的化学结构和组成;元素分析结果表明实验组的碳元素质量比高于对照组;与对照组相比,实验组能够明显促进碱性磷酸梅(ALP)的表达。结论采用仿生钙磷涂层法可在粗化纯钛表面成功构建含丹参涂层。
Objective To study the feasibility of assembly of Salvia miltiorrhiza Bge on bovine pure titanium using biomimetic calcium phosphate coating. Methods The surface of titanium plate was roughened. Different concentrations of Salvia miltiorrhiza coating were established on the surface of pure titanium by using bionic calcium phosphate coating. The surface morphology of the plate was characterized by field emission scanning electron microscopy (FSEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction ) And elemental analyzer to observe the crystal morphology, chemical structure and composition of the coating, and to verify its biological activity through cell culture. Results FSEM confirmed that Salvia miltiorrhiza had little effect on the crystal growth of Ca-P coating. Salvia miltiorrhiza had a greater effect on the crystal growth of Ca-P coating. XRD and FTIR showed that Salvia miltiorrhiza did not destroy the chemical structure and composition of coating . The results of elemental analysis showed that the carbon mass ratio of the experimental group was higher than that of the control group. Compared with the control group, the experimental group could significantly promote the expression of alkaline phosphatase (ALP). Conclusion Bismuth -ophthalide coating can be successfully constructed on the surface of rough titanium by biomimetic calcium phosphate coating.