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目的:研究骨愈搽剂中总黄酮及其单体成分的体外透皮行为。方法:采用垂直式Franz扩散池法,以离体小鼠皮肤为透皮屏障进行试验。采用紫外分光光度法测定总黄酮含量,高效液相色谱法测定单体黄酮羟基红花黄色素A(HSYA)含量,考察二者12 h内的体外透皮情况。结果:骨愈搽剂中总黄酮和HSYA的累积渗透量Q均随时间t的增加而增加,并与t1/2有显著相关性,透皮吸收过程均符合Higuchi方程(r=0.995 6、0.999 5),透过速率分别为126.24、47.516μg/(cm2·h)。结论:骨愈搽剂中总黄酮的透皮行为与HSYA的透皮行为具有相似性,均属于骨架扩散型透皮给药系统,具有长效缓释的特点。
Objective: To study the in vitro transdermal behavior of total flavonoids and their monomer components in Guyu liniment. Methods: The vertical Franz diffusion cell method was used to test the in vitro mouse skin as a transdermal barrier. The content of total flavonoids was determined by UV spectrophotometry and the content of monomer HSYA was determined by high performance liquid chromatography (HPLC). The in vitro transdermal conditions were observed within 12 hours. Results: The cumulative osmolality of total flavonoids and HSYA in Guyu lini increased with the increase of time t and was significantly correlated with t1 / 2. The transdermal absorption process was in accordance with Higuchi equation (r = 0.995 6, 0.999 5), the transmission rates were 126.24,47.516μg / (cm2 · h) respectively. CONCLUSION: The transdermal behavior of total flavonoids in Guyu lini is similar to that of HSYA, which belongs to the matrix diffusion transdermal delivery system and has the characteristics of long-term sustained release.