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背景与目的:姜黄素(curcumin)是中药姜黄的主要成分,其选择性的抑制肿瘤细胞增殖并诱导其凋亡。干扰素-α是目前用于临床治疗最重要的细胞因子之一。本研究旨在探讨干扰素-α联合姜黄素对B-NHL淋巴瘤Raji细胞的诱导凋亡作用及其作用机制。方法:以浓度2000u/L干扰素-α分别与6.25、12.5、25μmol/L浓度的姜黄素作用于体外培养的Raji细胞,观察细胞生长状态变化。MTT法检测细胞生长的抑制率,应用流式细胞仪法观察细胞凋亡,Western blot法检测24h,姜黄素(IC5025μmol/L)以及联合干扰素-α(2000u/L)对caspase6、caspase8、caspase9蛋白的影响。结果:干扰素-α与姜黄素联合应用可显著抑制Raji细胞的生长,诱导细胞发生凋亡,两者具有协同效应。凋亡蛋白caspase6、caspase8、caspase9表达显著增高,其作用呈现出明显的量效与时效关系。结论:干扰素-α与姜黄素联合应用对B-NHL淋巴瘤Raji细胞具有明显的增生抑制作用;促进凋亡蛋白的表达及诱导细胞凋亡,可能是其重要作用机制之一。
BACKGROUND & AIM: Curcumin is the main component of Chinese traditional medicine Turmeric, which selectively inhibits tumor cell proliferation and induces apoptosis. Interferon-α is currently one of the most important cytokines for clinical treatment. This study aimed to investigate the interferon-α combined with curcumin on B-NHL lymphoma Raji cells induced apoptosis and its mechanism of action. Methods: Curcumin at a concentration of 2000u / L interferon-α and 6.25, 12.5 and 25μmol / L respectively were used to effect the growth of Raji cells in vitro. The inhibitory rate of cell growth was detected by MTT assay. The apoptosis of cells was observed by flow cytometry. The expressions of caspase 6, caspase 8, caspase 9 and caspase 9 were detected by Western blot after 24h, curcumin (IC5025μmol / L) and interferon-α The impact of protein. Results: The combination of interferon-α and curcumin could significantly inhibit the growth of Raji cells and induce the apoptosis of the cells, both of which had a synergistic effect. The expressions of caspase6, caspase8 and caspase9 were significantly increased, and their effects were dose-dependent and time-dependent. Conclusion: The combination of interferon-α and curcumin can inhibit the proliferation of B-NHL lymphoma Raji cells obviously. Promoting the expression of apoptosis protein and inducing cell apoptosis may be one of the important mechanisms.